Go to main contentGo to navigation menu
University of Catania  >  Biometec
Reserved Area
Logo UniCt

Degree Course in

Biotechnology

Search

BIOCHIMICA E BIOLOGIA MOLECOLARE APPLICATA ALLE BIOTECNOLOGIE FARMACEUTICHE
Module BIOLOGIA MOLECOLARE APPLICATA

Academic Year 2025/2026 - Teacher: SIMONA REINA

Expected Learning Outcomes

The course Applied Molecular Biology for Pharmaceutical Sciences provides the theoretical and practical foundations of modern molecular biotechnology. The curriculum explores recombinant DNA technology (vectors and cloning), nucleic acid extraction techniques, agarose electrophoresis, and the evolution of sequencing systems (from first to third generation). Special focus is given to the production of recombinant proteins and the development of biopharmaceutical drugs, such as vaccines and monoclonal antibodies. Furthermore, the course covers gene expression analysis and the latest frontiers in genome editing through transgenics and the CRISPR-Cas9 system.
By the end of the course, students will have acquired the ability to integrate these methodologies for experimental design and industrial production, understanding the therapeutic value of biological macromolecules within the pharmaceutical sector.

Course Structure

The course consists of frontal lectures (face-to-face teaching) and includes 1 CFU of practical training held in the Molecular Biology Laboratory.

Required Prerequisites

To successfully attend the course, students are required to have a basic knowledge of biochemistry and molecular biology.

Attendance of Lessons

Attendance is mandatory

Detailed Course Content

The course includes:

DNA Manipulation: DNA extraction and purification from cells, DNA visualization via agarose gel electrophoresis. PCR and its variants: Multiplex, mutagenesis, nested, RT-PCR, Real-time PCR (SYBR green, molecular beacons, scorpions). Gene Cloning: Restriction enzymes, cloning and expression vectors, strategies for creating recombinant molecules. Genomic Sequencing: First-generation techniques (Sanger), Next-Generation Sequencing (NGS), and third-generation sequencing (Nanopore, PacBio). Genome Editing: Creation of transgenic organisms and application of the CRISPR-Cas9 system in pharmaceutical research. Recombinant Protein Production: Production strategies in host systems (bacteria, yeast, mammals). Immunological Techniques: ELISA, Western blot. Biopharmaceutical Drugs: Design and production of recombinant vaccines and monoclonal antibodies. Gene Expression Analysis: Techniques for transcriptome study: EMSA, REMSA, Luciferase assay, ChIP, overview of RNAseq and methylome.

Learning Assessment

Learning Assessment Procedures

Oral interview

Information for students with disabilities and/or DSA

To guarantee equal opportunities and in compliance with current laws, interested students can request a personal interview in order to plan any compensatory and/or dispensatory measures, based on the educational objectives and specific needs. It is also possible to contact the CInAP representative (Centre for Active and Participatory Integration - Services for Disabilities and/or DSA) of the Department of Biomedical and Biotechnological Sciences.

VERSIONE IN ITALIANO