ADVANCED MOLECULAR AND EXPERIMENTAL HEMATOLOGY

Academic Year 2025/2026 - Teacher: ALESSANDRA ROMANO

Expected Learning Outcomes

Acquisition of the basic knowledge necessary for the choice and use of omics for the diagnosis and attribution of the risk of progression of the main neoplastic hemopathies.

At the end of the course, students will be able to choose and develop the most suitable technological approach for understanding the pathophysiological basis of hematological neoplasms, with particular reference to multiple myeloma, non-Hodgkin's lymphomas and chronic lymphocytic leukemia.

Course Structure

After a general overview of the main omics technologies developed for understanding the main hematological neoplasms, the student will be introduced to the application of gene therapy, immunotherapy and metabolomics used for the development of precision medicine. At each lesson, the discussion of a scientific article will make it possible to critically highlight the experimental approach adopted for understanding the pathology of interest.

Required Prerequisites

According to Regolamento di Ateneo. Basic knowledge of the HLA system, pathophysiology of chronic and neoplastic inflammation processes, anatomy of the immune system is suggested, molecular hematology

Attendance of Lessons

Attendance is highly recommended due to the interactive nature of the lessons.

Detailed Course Content

Through the discussion of the most recent scientific articles, examples of application of the most advanced biotechnologies in the field of onco-haematological research will be proposed for the diagnosis and monitoring of minimal residual disease in the main haematological neoplasms and to evaluate the efficacy over time of cellular and genic.

Textbook Information

Author	Title	Publisher	Year	ISBN
Bosi, De Stefano, Di Raimondo, La Nasa	Manuale di malattie del Sangue	Elsevier	2012	9788821432620
Pier Luigi Zinzani, Sante Tura, Michele Cavo	Hematology. Pathophysiology, diagnosis and treatment	Esculapio	2018	8893850834

Learning Assessment

Learning Assessment Procedures

Learners will be invited to create a systemic diagram to illustrate one or more strategies with which to deal with resistance to one of the cellular therapies covered by the course. There is an ongoing test with 30 multiple choice questions for the intermediate assessment of learning.

Examples of frequently asked questions and / or exercises

What are NETs (neutrophil extracellular traps)?

A. Filaments of nuclear material (DNA, histones, and chromatin fibers) released by neutrophil granulocytes after contact with a

monocyte/macrophage.

B. They are fibrillar structures released following the necrosis of basophil granulocytes that have undergone apoptosis.

C. They are extracellular fibrillar networks that allow a high concentration of antimicrobial substances to reach sites of infection.

D. They are structures formed by granulocytes and macrophages to trap and destroy microorganisms.

VERSIONE IN ITALIANO

Degree Course in

Medical Biotechnologies