MOLECULAR ONCOLOGY AND IMMUNOPATHOLOGY APPLIED TO HUMAN BIOTECHNOLOGY

Module IMMUNOLOGY TECHNIQUES APPLIED TO HUMAN BIOTECHNOLOGY

The specific objectives of the course are to allow the student to acquire the fundamental notions on the diagnostic methods for the evaluation of immune system pathologies.

Taking the Dublin descriptors into account, the expected learning outcomes are:

1. Knowledge and understanding: At the end of the module, students will have acquired theoretical and scientific knowledge related to the principles of applied immunology and the main diagnostic methods used to evaluate immune system disorders. Specifically, they will understand the basics of immunological techniques and their use in the diagnosis of immune-mediated pathological conditions.

2. Ability to apply knowledge and understanding: Students will be able to apply their acquired knowledge to the interpretation of results from the main immunodiagnostic techniques (such as ELISA, immunofluorescence, and flow cytometry), understanding their purposes, methods of use, and limitations. They will also be able to correlate laboratory results with the main immune system alterations.

3. Independent judgment: Students will have developed critical skills in evaluating data obtained using immunological techniques, being able to recognize the reliability of results, methodological limitations, and potential sources of error. They will be able to critically interpret diagnostic information in the context of immune disorders.

4. Communication skills: Students will be able to clearly and appropriately communicate their knowledge of immunological methods and their fields of application, using appropriate scientific terminology. They will be able to describe the principles and results of immunodiagnostic tests to both specialists and non-specialists.

5. Learning skills: Students will have developed independent learning skills that will allow them to continuously update their knowledge in the field of applied immunology and diagnostic techniques, including through critical consultation of scientific literature, in a rapidly evolving technological field.

Polyclonal and monoclonal antibodies. Production techniques: Hybridomas and Phage Display

ELISA: direct, indirect, sandwich and competitive ELISAs; Standard curve and interpolation of unknown values

Flow cytometric techniques: basics; compensation; cell cycle study; analysis of apoptosis

Immunofluorescence: principles and applications in the diagnosis of autoimmune diseases

Sierology: principles and applications

Transplant immunology

Abbas, Lichtman, Pillai. Immunologia cellulare e molecolare. Edra; 9° edizione 2018

Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019