MOLECULAR ONCOLOGY AND IMMUNOPATHOLOGY APPLIED TO HUMAN BIOTECHNOLOGYModule TECNICHE DI IMMUNOLOGIA APPLICATE ALLE BIOTECNOLOGIE UMANE
Academic Year 2022/2023 - Teacher: PAOLO FABRIZIO FAGONEExpected Learning Outcomes
The specific objectives of the course are to allow the student to acquire the fundamental notions on the diagnostic methods for the evaluation of immune system pathologies
Course Structure
Required Prerequisites
Attendance of Lessons
Detailed Course Content
Polyclonal and monoclonal antibodies. Production techniques: Hybridomas and Phage Display
ELISA: direct, indirect, sandwich and competitive ELISAs; Standard curve and interpolation of unknown values
Flow cytometric techniques: basics; compensation; cell cycle study; analysis of apoptosis
Immunofluorescence: principles and applications in the diagnosis of autoimmune diseases
Sierology: principles and applications
Transplant immunology
Textbook Information
Abbas, Lichtman, Pillai. Immunologia cellulare e molecolare. Edra; 9° edizione 2018
Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Anticorpi monoclonali | Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019; Abbas, Lichtaman, Pillai. Immunologia cellulare e molecolare. Edra; 9° edizione 2018 |
| 2 | ELISA e immunofluorescenza | McPherson. HENRY'S Diagnosi Clinica e Metodi di Laboratorio. Antonio Delfino Editore; Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019 |
| 3 | Citofluorimetria | McPherson. HENRY'S Diagnosi Clinica e Metodi di Laboratorio. Antonio Delfino Editore; Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019 |
| 4 | Sierologia | McPherson. HENRY'S Diagnosi Clinica e Metodi di Laboratorio. Antonio Delfino Editore; Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019 |
| 5 | Immunolgia dei trapainti | McPherson. HENRY'S Diagnosi Clinica e Metodi di Laboratorio. Antonio Delfino Editore; Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019 |